Journal: Journal of Clinical Pathology

Article Title: Unusual fusion gene rearrangements in patients with nodular fasciitis: a study of rare and novel USP6 fusion partners with a review of the literature

doi: 10.1136/jcp-2023-208768

Figure Lengend Snippet: Patient characteristics and clinical findings in USP6::MYH9 fusion cases

Article Snippet: Thin histological sections (3 μm thick) were used, and each sample was stained using the following antibodies and protocols: anti-smooth muscle actin (SMA) mouse monoclonal antibodies (clone 1A4, BioSB— Bioscience for the World, dilution 1:75; pretreatment: heating up to 99°C in a pH 6 buffer in a water bath); anti-H-caldesmon mouse monoclonal antibodies (clone BSB-19|, BioSB, dilution 1:100; pretreatment: heating up to 99°C in a pH 9 buffer in a water bath); anti-desmin mouse monoclonal antibodies (clone D33, BioSB, dilution 1:100; pretreatment: heating up to 99°C in a pH 9 buffer in a water bath); anti-S100-β rabbit monoclonal antibodies (clone EP32, BioSB, dilution 1:300; pre-treatment: heating up to 99 ◦C in a pH 9 buffer in a water bath); anti-Ki-67 mouse monoclonal antibodies (clone MIB-1, BioSB, dilution 1:150; pretreatment: heating up to 99°C in a pH 6 buffer in a water bath).

Techniques:

Journal: Molecules

Article Title: Targeting Chondroitin Sulphate Synthase 1 (Chsy1) Promotes Axon Growth Following Neurorrhaphy by Suppressing Versican Accumulation

doi: 10.3390/molecules28093742

Figure Lengend Snippet: Confocal photomicrographs of the distribution of S100 and Chsy1 in regenerating axons at one and three months following ESN. Arrow indicated S100-positive cells. Arrow head indicated distribution of Chsy1.The recipient nerve tissue (distal end of McN) was immunostained with anti-S100 antibody (green) and anti-Chsy1 antibody (red) in the sham operation group ( A – C ), ES1M-saline group ( D – F ), and ES3M-saline group ( G – I ). After labeling the sections, nuclei DNA were counterstained with Hoechst33342 (blue). S100-positive Schwann cell and Chsy1 expression appear in the regenerating axons of ES1M group. Scale bar = 25 μm. Immunoblots ( J ) and histogram ( K ) showing versican V1, Chsy1, and S100 expressions in the nerve tissues of sham-operated and 1- to 3-month ESN rats. β-actin was used as a loading control. Data are present as mean ± SD from three independent experiments. * p < 0.05, compared with the sham operation group. # p < 0.05, compared with the ES1M si-ctrl group.

Article Snippet: After the protein blocking step, the sections were incubated in rabbit polyclonal anti-S100 antibody (1:1000, Taiclone Biotech Corp, Taipei, Taiwan), rabbit polyclonal anti-Chsy1 antibody (1:1000, Origene, Rockville, MD, USA), rabbit polyclonal anti-versicanV 1 antibody (1:500, Invitrogen, ThermoFisher, Waltham, MA, USA), and rabbit polyclonal anti-β3-tubulin antibody (1:1000, Proteintech, Manchester, UK), with the blocking solution for 24 h at 4 °C.

Techniques: Saline, Labeling, Expressing, Western Blot, Control

Journal: Molecules

Article Title: Targeting Chondroitin Sulphate Synthase 1 (Chsy1) Promotes Axon Growth Following Neurorrhaphy by Suppressing Versican Accumulation

doi: 10.3390/molecules28093742

Figure Lengend Snippet: Protein expressions in the recipient nerve tissue one month after the siRNA treatment. ( A ) Western blots showing versican V1 expression and S100, PGP9.5, and β3-tubulin distribution in the sham operation, ES1M si-ctrl, and ES1M si-Chsy1 groups. ( B ) Histogram showing the expression of each protein in the sham-op, ES1M si-ctrl, and ES1M si-Chsy1 groups. β-Actin was used as a loading control. Data are present as mean ± standard deviation from three independent experiments. * p < 0.05, compared to the sham operation group. # p < 0.05, compared to the ES1M si-ctrl group.

Article Snippet: After the protein blocking step, the sections were incubated in rabbit polyclonal anti-S100 antibody (1:1000, Taiclone Biotech Corp, Taipei, Taiwan), rabbit polyclonal anti-Chsy1 antibody (1:1000, Origene, Rockville, MD, USA), rabbit polyclonal anti-versicanV 1 antibody (1:500, Invitrogen, ThermoFisher, Waltham, MA, USA), and rabbit polyclonal anti-β3-tubulin antibody (1:1000, Proteintech, Manchester, UK), with the blocking solution for 24 h at 4 °C.

Techniques: Western Blot, Expressing, Control, Standard Deviation